Electrochemical Reduction of Protein/Peptide Disulfide Bonds

Scheme 3. Top-down characterization of proteins after online disuflide-bond reduction¹¹

Traditional methods for protein structural analysis involves chemical reduction of disulfide bonds prior to mass spectrometric detection, which is time-consuming. We have developed online electrochemical reduction approach^10-13 which is reagentless and fast, facilitating MS elucidation of protein structures for proteomic research.

For instance, after online electrochemical reduction of disulfide bonds, sequence coverage from MS/MS dissociation of resulting reduced protein ions (Scheme 3) are greatly improved.

The online electrochemical reduction of disulfde bond can be also used to assist cross-linking MS to probe protein/protein interactions and protein 3D structures.^14-15

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