The central aims of our research are to develop methodologies and “rules” for enzyme design and apply these methods to efficiently create novel and practical biocatalysts for applications in drug design, bioremediation, biofuels and green chemistry synthesis. We are currently engineering proteins for these biocatalysts using directed evolution and rational approaches. Our goals are developing high-throughput screening technologies to assay mutant enzyme libraries to discover novel biocatalysts; combining rational and directed evolution approaches to create de novo enzymes; engineering and designing metabolic pathways in bacteria for synthetic biology; and developing novel protein display technologies.